4FWG
Crystal structure of the Lon-like protease MtaLonC in complex with lactacystin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-10 |
Detector | BRUKER SMART 6500 |
Wavelength(s) | 1.0 |
Spacegroup name | P 6 |
Unit cell lengths | 115.987, 115.987, 135.054 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.990 |
R-factor | 0.23101 |
Rwork | 0.230 |
R-free | 0.25893 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fw9 |
RMSD bond length | 0.030 |
RMSD bond angle | 2.673 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.160 | 0.784 |
Number of reflections | 75772 | |
<I/σ(I)> | 13.4 | 2.7 |
Completeness [%] | 99.5 | 100 |
Redundancy | 8.5 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10% isopropanol, 200mM di-potassium phosphate, 100mM sodium citrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |