4FVU

Structural basis for the dsRNA specificity of the Lassa virus NP exonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	44.772, 84.311, 79.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.904 - 2.910
R-factor	0.1996
Rwork	0.197
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.330
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.904
High resolution limit [Å]	2.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M Bis-Tris pH 6.5, 0.2 M MgCl2 hexahydrate and 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K