4FVJ
SPFH domain of the mouse stomatin (Crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-08 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918410 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.530, 91.380, 86.138 |
| Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
| Resolution | 82.760 - 2.690 |
| R-factor | 0.24783 |
| Rwork | 0.246 |
| R-free | 0.29009 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fvf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.760 | 2.740 |
| High resolution limit [Å] | 2.670 | 2.690 |
| Number of reflections | 32523 | |
| <I/σ(I)> | 6.8 | 2.7 |
| Completeness [%] | 91.3 | 76.2 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 7% ethanol, 5% glycerol, 0.8 M sodium acetate, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
