4FVB
Crystal structure of EV71 2A proteinase C110A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-11-03 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.613, 44.265, 52.036 |
Unit cell angles | 90.00, 112.58, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hrv |
RMSD bond length | 0.023 |
RMSD bond angle | 2.024 |
Data reduction software | AUTOMAR |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.035 | 0.262 |
Number of reflections | 13431 | |
<I/σ(I)> | 11.9 | 2.1 |
Completeness [%] | 92.4 | 96 |
Redundancy | 1.93 | 1.84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 100mM HEPES, 20% 2-propanol, 10% PEG 4000, pH 7.5, vapor diffusion, hanging drop, temperature 289K |