4FVA
Crystal structure of truncated Caenorhabditis elegans TDP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-12-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.600, 104.400, 84.700 |
| Unit cell angles | 90.00, 90.98, 90.00 |
Refinement procedure
| Resolution | 44.993 - 2.070 |
| R-factor | 0.1606 |
| Rwork | 0.159 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.106 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1066)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.066 | 0.566 |
| Number of reflections | 66104 | |
| <I/σ(I)> | 14.16 | 3.5 |
| Completeness [%] | 99.1 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 293 | 30% PEG 3,350 and 0.2M sodium malonate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
