4FUS
The X-ray structure of Hahella chejuensis family 48 glycosyl hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-11 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.690, 78.389, 67.075 |
| Unit cell angles | 90.00, 98.94, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.750 |
| R-factor | 0.15639 |
| Rwork | 0.154 |
| R-free | 0.20466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9g |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.069 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 63636 | |
| <I/σ(I)> | 12.71 | 1.93 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.47 | 3.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294 | 0.05 M potassium phosphate monobasic and 20 % (w/v) polyethylene glycol 8000, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
