4FUQ
Crystal structure of apo MatB from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979206 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.872, 58.813, 138.957 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 24.909 - 1.702 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19670 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.089 | 0.080 | 0.483 |
| <I/σ(I)> | 33.96 | 3.5 | |
| Completeness [%] | 97.1 | 97.8 | 95.5 |
| Redundancy | 4.2 | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100 mM Bis-Tris pH 6.5, 84 mM MgSO4, 19.5 % (w/v) monomethyl polyethylene glycol 2000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
