4FUL
PI3 Kinase Gamma bound to a pyrmidine inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.040, 66.900, 102.820 |
| Unit cell angles | 90.00, 97.59, 90.00 |
Refinement procedure
| Resolution | 33.340 - 2.470 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.900 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.064 | 0.638 |
| Number of reflections | 34136 | |
| <I/σ(I)> | 13.7 | 2.2 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 100 mM tris HCl pH8.3, 100 mM Ammonium Sulfate, 18% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
