4FUK

Aminopeptidase from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	48.385, 75.545, 180.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.190 - 1.750
R-factor	0.19593
Rwork	0.194
R-free	0.22891
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g6p
RMSD bond length	0.011
RMSD bond angle	1.382
Data reduction software	XDS
Data scaling software	SCALA (CCP4_3.3.20)
Phasing software	PHASER
Refinement software	REFMAC (5.7.0027)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.390	48.390	1.840
High resolution limit [Å]	1.750	5.530	1.750
Rmerge	0.085	0.040	0.790
Number of reflections	67991
<I/σ(I)>	13.8205
Completeness [%]	100.0	99.88	99.97
Redundancy	7.36	6.67	7.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	0.1M Tris pH 6.5, 28% peg 2K MME, vapor diffusion, temperature 298K