4FTH
Crystal Structure of NtrC4 DNA-binding domain bound to double-stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-04-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.436, 28.798, 74.931 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 48.108 - 3.004 |
| R-factor | 0.2452 |
| Rwork | 0.243 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e7l |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.802 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.063 | |
| Number of reflections | 5777 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 96.8 | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 30 mM Na-cacodylate pH 6.0, 140 mM spermidine, 50 mM potassium thiocyanate, and 15% (poly)ethylene glycol mw 400, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
