4FS5
Crystal structure of the Z-DNA hexamer CGCGCG at 500 mM MgCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 18.593, 18.593, 72.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.682 - 1.300 |
| R-factor | 0.2133 |
| Rwork | 0.211 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1io5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.849 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.200 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.054 | 0.120 |
| Number of reflections | 6868 | |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | TEMPERATURE CONTROL | 7 | 293 | 2.0mM DNA, 20mM Na cacodylate, 30% 2-methyl-2,4-pentainediol, 500mM MgCl2, pH 7.0, TEMPERATURE CONTROL, temperature 293K |
