4FRT
Crystal Structure of Pseudomonas aeruginosa OccK7 (OpdD)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.250, 111.154, 99.933 |
| Unit cell angles | 90.00, 123.25, 90.00 |
Refinement procedure
| Resolution | 14.989 - 3.166 |
| R-factor | 0.2127 |
| Rwork | 0.210 |
| R-free | 0.26870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t24 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.395 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 23348 | |
| <I/σ(I)> | 18 | 3.55 |
| Completeness [%] | 99.1 | 98.8 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG 400, 1M sodium formate, 0.05M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
