4FQT
Structure of AgamOBP1 Bound to 6-methyl-5-hepten-2-one
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.769, 68.993, 64.552 |
| Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
| Resolution | 31.697 - 2.200 |
| R-factor | 0.1877 |
| Rwork | 0.185 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.413 |
| Data reduction software | d*TREK (9.7L) |
| Data scaling software | d*TREK (9.7L) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.260 | 46.260 | 1.630 |
| High resolution limit [Å] | 1.570 | 3.380 | 1.570 |
| Rmerge | 0.151 | 0.074 | 0.750 |
| Number of reflections | 26439 | ||
| <I/σ(I)> | 4.5 | 11.4 | 0.3 |
| Completeness [%] | 68.4 | 99.3 | 3.8 |
| Redundancy | 3.17 | 3.74 | 1.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
