4FQR
Crystal structure of broadly neutralizing antibody C05 bound to H3 influenza hemagglutinin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 142.020, 158.620, 178.500 |
| Unit cell angles | 89.95, 85.44, 84.37 |
Refinement procedure
| Resolution | 49.070 - 4.100 |
| R-factor | 0.3105 |
| Rwork | 0.309 |
| R-free | 0.33360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4FNK AND 4FNL |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.335 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.200 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Number of reflections | 89665 | |
| <I/σ(I)> | 5.3 | 1.9 |
| Completeness [%] | 78.7 | 53.4 |
| Redundancy | 1.5 | 0.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 28% w/v PEG400, 200 mM calcium chloride, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
