4FQQ

Crystal Structure of Germline Antibody PGT121-GL Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	54.934, 344.743, 55.230
Unit cell angles	90.00, 91.95, 90.00

Refinement procedure

Resolution	37.440 - 2.420
R-factor	0.195
Rwork	0.194
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.951
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.305	38.305	2.550
High resolution limit [Å]	2.419	7.650	2.420
Rmerge		0.028	0.482
Number of reflections	74318
<I/σ(I)>	11	22	1.6
Completeness [%]	95.5	96.2	80.1
Redundancy	3.1	3.1	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	PEG 3350, sodium malonate, MnCl2, pH 7.0, vapor diffusion, temperature 298K