4FQP
Crystal structure of human Nectin-like 5 full ectodomain (D1-D3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 138.919, 138.919, 273.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.600 |
| R-factor | 0.24953 |
| Rwork | 0.248 |
| R-free | 0.27248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3EOW |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.761 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.730 |
| High resolution limit [Å] | 3.563 | 3.563 |
| Number of reflections | 19387 | |
| <I/σ(I)> | 13.7 | 2.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.3 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 55% v/v tacsimate, 0.1 M Bicine, pH 9.0, with additional 10% tacsimate as cryoprotectant, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
