4FQL

Influenza B HA Antibody (Fab) CR8033

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	136.200, 55.000, 80.800
Unit cell angles	90.00, 121.20, 90.00

Refinement procedure

Resolution	40.245 - 1.898
R-factor	0.1962
Rwork	0.194
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n9g
RMSD bond length	0.007
RMSD bond angle	1.177
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.113	2.000
High resolution limit [Å]	1.898	1.898
Number of reflections	40203
<I/σ(I)>	6.9	2.6
Completeness [%]	98.0	98.2
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	200 mM zinc acetate, 10% PEG8000, 100 mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K