4FQJ

Influenza B/Florida/4/2006 hemagglutinin Fab CR8071 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Detector technology	CCD
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	45.570, 111.930, 168.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.597 - 2.500
R-factor	0.2323
Rwork	0.230
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2RFT AND 3N9G
RMSD bond length	0.009
RMSD bond angle	1.287
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	30377
<I/σ(I)>	15.6	2.4
Completeness [%]	98.9	99.2
Redundancy	5.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	20% PEG3350, 200 mM sodium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K