4FQE
KdgM porin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.856, 59.674, 51.564 |
| Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.930 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wjq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.503 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.3.16 2010/01/06) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.360 | 49.360 | 2.030 |
| High resolution limit [Å] | 1.930 | 6.100 | 1.930 |
| Rmerge | 0.073 | 0.030 | 0.390 |
| Total number of observations | 1933 | 8662 | |
| Number of reflections | 16187 | ||
| <I/σ(I)> | 10.4549 | 18.76 | 1.57 |
| Completeness [%] | 99.9 | 98.65 | 99.93 |
| Redundancy | 3.78 | 3.64 | 3.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES, 22% MPD, 36mM digalacturonate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
