4FPV
Crystal structure of D. rerio TDP2 complexed with single strand DNA product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 2011-12-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.070, 95.021, 104.506 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.150 - 1.730 |
| R-factor | 0.15869 |
| Rwork | 0.159 |
| R-free | 0.20159 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 60160 | |
| Completeness [%] | 96.5 | 89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 30% PEG 3,350 and 0.2M sodium tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
