4FP9
Human MTERF4-NSUN4 protein complex
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.760, 82.270, 507.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.2295 |
| Rwork | 0.229 |
| R-free | 0.24610 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 | |
| High resolution limit [Å] | 2.900 | 40.000 | 2.900 |
| Rmerge | 0.105 | 0.020 | 0.977 |
| Number of reflections | 74514 | 11 | 7096 |
| <I/σ(I)> | 11.58 | 45.94 | 1.76 |
| Completeness [%] | 99.8 | 27.5 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 294 | 100 mM Bis-Tris [pH 5.5], 400 mM ammonium sulfate, 20-22 % (w/v) polyethylene glycol 3350, and 2 mM dithiothreitol, VAPOR DIFFUSION, temperature 294K |
