4FOL
S-formylglutathione hydrolase Variant H160I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-07 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.300, 119.740, 169.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.730 - 2.070 |
| R-factor | 0.28316 |
| Rwork | 0.281 |
| R-free | 0.32674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pv1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.319 |
| Data reduction software | SAINT |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 69.000 |
| High resolution limit [Å] | 2.070 |
| Number of reflections | 75437 |
| <I/σ(I)> | 5.49 |
| Completeness [%] | 90.9 |
| Redundancy | 4.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 0.17 M ammonium acetate, 0.085 M sodium acetate trihydrate, pH 4.6, 25.5% PEG4000, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
