4FO0
Human actin-related protein Arp8 in its ATP-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97641 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.875, 151.262, 173.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.897 - 2.600 |
| R-factor | 0.1657 |
| Rwork | 0.163 |
| R-free | 0.20950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qb0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.590 |
| Rmerge | 0.787 | |
| Number of reflections | 32838 | |
| <I/σ(I)> | 2.17 | |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 289 | 3.9 M NaCl, 0.5 % (v/v) methanol, 50 mM Mes pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
