4FMW
Crystal structure of methyltransferase domain of human RNA (guanine-9-) methyltransferase domain containing protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-16 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97903 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.097, 43.309, 60.347 |
| Unit cell angles | 100.43, 90.03, 92.88 |
Refinement procedure
| Resolution | 37.990 - 2.000 |
| R-factor | 0.2193 |
| Rwork | 0.218 |
| R-free | 0.24330 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.104 | 0.571 |
| Number of reflections | 24649 | |
| <I/σ(I)> | 22.5 | 3.42 |
| Completeness [%] | 97.9 | 96.4 |
| Redundancy | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297 | 2.22 M NH4SO4, 0.1 M Tris-HCl, 10% Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
