4FMQ

Crystal structure of human ERK2 complexed with a MAPK docking peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-21
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.032, 65.657, 117.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.037 - 2.098
R-factor	0.1824
Rwork	0.180
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gph
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.037	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	18465
Completeness [%]	88.1	90.4
Redundancy		4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	20-24% PEG6000, 100mM TRIS, pH 8.5, VAPOR DIFFUSION, temperature 298K