4FMQ
Crystal structure of human ERK2 complexed with a MAPK docking peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-21 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.032, 65.657, 117.228 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.037 - 2.098 |
R-factor | 0.1824 |
Rwork | 0.180 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gph |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.037 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 18465 | |
Completeness [%] | 88.1 | 90.4 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 20-24% PEG6000, 100mM TRIS, pH 8.5, VAPOR DIFFUSION, temperature 298K |