4FMO
Structure of the C-terminal domain of the Saccharomyces cerevisiae MUTL alpha (MLH1/PMS1) heterodimer bound to a fragment of exo1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.700, 66.140, 74.470 |
| Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
| Resolution | 48.410 - 3.040 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e4w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.230 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.136 | 0.793 |
| Number of reflections | 18219 | |
| <I/σ(I)> | 10 | 2.2 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
