4FMG
Merkel Cell Polyomavirus VP1 Unassembled Pentamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 85.270, 85.620, 248.170 |
| Unit cell angles | 92.98, 100.50, 108.05 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.18277 |
| Rwork | 0.182 |
| R-free | 0.22699 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.113 | 0.569 |
| Number of reflections | 351188 | |
| <I/σ(I)> | 8.98 | 1.95 |
| Completeness [%] | 92.3 | 64.7 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium cacodylate, pH 6.5, 6% w/v PEG3350, 0.3 M magnesium chloride, cryoprotection: 25% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
