4FM2
Pyrococcus abyssi B family DNA polymerase (triple mutant) bound to a dsDNA, in edition mode
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.919360 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.730, 115.020, 127.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.110 - 2.900 |
| R-factor | 0.1845 |
| Rwork | 0.183 |
| R-free | 0.22150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4flw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.110 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.920 |
| Rmerge | 0.104 | 0.860 |
| Number of reflections | 21903 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 97.7 | 98.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 7-12% PEG 20000, 100 mM MES pH 6.5, seeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
