4FLH
Crystal structure of human PI3K-gamma in complex with AMG511
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-13 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.980 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.059, 67.803, 107.523 |
| Unit cell angles | 90.00, 95.27, 90.00 |
Refinement procedure
| Resolution | 41.150 - 2.600 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.24430 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.680 |
| High resolution limit [Å] | 2.590 | 5.580 | 2.590 |
| Rmerge | 0.052 | 0.027 | 0.561 |
| Number of reflections | 32540 | ||
| <I/σ(I)> | 16.3 | ||
| Completeness [%] | 99.8 | 99 | 100 |
| Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 21% PEG 3350, 200 mM ammonium sulfate, 2 mM DTT, 100 mM Tris, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
