4FLB
CID of human RPRD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-15 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.339, 40.339, 145.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.540 - 1.802 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21620 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | SCALA (CCP4_3.3.20) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.540 | 28.520 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.700 | 1.800 |
| Rmerge | 0.093 | 0.030 | 0.750 |
| Total number of observations | 4239 | 20732 | |
| Number of reflections | 11740 | ||
| <I/σ(I)> | 25.0113 | ||
| Completeness [%] | 98.9 | 98.63 | 97.08 |
| Redundancy | 12.46 | 9.34 | 12.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 291 | 25% PEG-3350, 0.2M ammounium sulfate, 0.1M sodium cacodylate, praseodymium(III) acetate, pH 5.5, vapor diffusion, temperature 291K |
