4FL6

Crystal structure of the complex of the 3-MBT repeat domain of L3MBTL3 and UNC1215

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Collection date	2012-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97931
Spacegroup name	C 1 2 1
Unit cell lengths	228.026, 54.372, 65.549
Unit cell angles	90.00, 97.11, 90.00

Refinement procedure

Resolution	44.110 - 2.550
R-factor	0.1981
Rwork	0.195
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	related to pdb entry 3UT1
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	SCALA (CCP4_3.3.20)
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.570	45.570	2.690
High resolution limit [Å]	2.550	8.060	2.550
Rmerge	0.062	0.020	0.980
Total number of observations		2991	12928
Number of reflections	26075
<I/σ(I)>	15.2968
Completeness [%]	99.2	97.45	98.3
Redundancy	3.68	3.43	3.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	291	25% PEG-3350, 0.2M ammonium acetate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, temperature 291K
1	VAPOR DIFFUSION	5.5	291	25% PEG-3350, 0.2M ammonium acetate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, temperature 291K