4FL4
Scaffoldin conformation and dynamics revealed by a ternary complex from the Clostridium thermocellum cellulosome
Replaces: 3P0DExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.374, 186.305, 191.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.947 - 2.800 |
| R-factor | 0.1932 |
| Rwork | 0.191 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.189 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.043 | 0.643 |
| Number of reflections | 104697 | |
| <I/σ(I)> | 22.27 | 2.1 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.8 | 3.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M HEPES, 1.3-1.5 M lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
