4FL1

Structural and Biophysical Characterization of the Syk Activation Switch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-11-26
Detector	PSI PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	40.083, 85.403, 92.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.400 - 1.790
R-factor	0.197
Rwork	0.192
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.400
High resolution limit [Å]	1.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES, 28% PEG 3350, 0.2 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K