4FJU
Crystal structure of ureidoglycolate dehydrogenase in ternary complex with NADH and glyoxylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-05 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 162.756, 162.756, 61.453 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.393 - 1.771 |
| R-factor | 0.2296 |
| Rwork | 0.229 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3QGR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.141 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.074 | 0.583 |
| Number of reflections | 74082 | |
| <I/σ(I)> | 46.7 | 4.6 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 15.7 | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 295 | 0.1M MES, 4.0M NACL, pH 6.0, EVAPORATION, temperature 295K |
