4FJ3

14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-02-14
Detector	PSI PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	72.140, 102.430, 113.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.730 - 1.950
R-factor	0.1611
Rwork	0.159
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.026
RMSD bond angle	1.724
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.6.0093)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	20.000	2.000
High resolution limit [Å]	1.950	10.000	1.950
Rmerge	0.052	0.034	0.436
Number of reflections	61869	523	4451
<I/σ(I)>	19.57	38.04	4.31
Completeness [%]	99.5	96.7	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M Na-acetate pH 7.0, 0.8 M NaH2PO4 and 1.2 M K2HPO4 , VAPOR DIFFUSION, SITTING DROP, temperature 277K