4FIH
Catalytic domain of human PAK4 with QKFTGLPRQW peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97550 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.673, 61.673, 181.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.970 |
| R-factor | 0.18085 |
| Rwork | 0.178 |
| R-free | 0.22527 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cdz |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.014 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.072 | 0.593 |
| <I/σ(I)> | 36.8 | 3.9 |
| Completeness [%] | 99.5 | 96.1 |
| Redundancy | 11.4 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M Tris-HCl, 1.5M Na acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
