4FHW
Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - MgGTP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.413, 62.897, 111.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.170 - 2.500 |
R-factor | 0.19905 |
Rwork | 0.196 |
R-free | 0.24893 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fh3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.169 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 13540 | |
<I/σ(I)> | 13.8 | 4.43 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.4 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 100 mM CHES, pH 9.5, 5-10% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |