4FHN

Nup37-Nup120 full-length complex from Schizosaccharomyces pombe

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	329.999, 329.999, 350.262
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	95.117 - 6.989
R-factor	0.2884
Rwork	0.285
R-free	0.34560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.267
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.117	95.117	7.230
High resolution limit [Å]	6.984	27.050	6.980
Rmerge		0.026	0.026
Total number of observations		5905	31522
Number of reflections	18313
<I/σ(I)>	15.2	11.4	0.3
Completeness [%]	99.5	95.5	95.9
Redundancy	22.4	16.2	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	100 mM Bis-Tris/HCl pH 5.75-6.5, 1.75-2.25 M NaCl , VAPOR DIFFUSION, HANGING DROP, temperature 291.0K