4FHL

Nucleoporin Nup37 from Schizosaccharomyces pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	131.726, 131.726, 117.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.553 - 2.600
R-factor	0.1769
Rwork	0.176
R-free	0.18970
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.834
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX (1.8_1063)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.553	46.553	2.690
High resolution limit [Å]	2.600	10.070	2.600
Rmerge		0.011	0.011
Total number of observations		4863	30168
Number of reflections	32471
<I/σ(I)>	16.9	15.4	0.7
Completeness [%]	100.0	98.6	100
Redundancy	9.5	7.5	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.75	303	100 mM Tris/HCl pH 7.75-8.25, 4% (v/v) 1,4-butanediol, 400-500 mM Li2SO4 , VAPOR DIFFUSION, HANGING DROP, temperature 303.0K