4FH0
Crystal Structure of Human BinCARD CARD, double mutant F16M/L66M SeMet form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537, 0.9797, 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.196, 36.669, 43.276 |
| Unit cell angles | 108.54, 90.30, 111.01 |
Refinement procedure
| Resolution | 27.724 - 1.400 |
| R-factor | 0.1395 |
| Rwork | 0.137 |
| R-free | 0.17720 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.303 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.139 | 1.470 |
| High resolution limit [Å] | 1.250 | 1.400 |
| Rmerge | 0.080 | 0.580 |
| Number of reflections | 45785 | |
| <I/σ(I)> | 16.2 | 4.4 |
| Completeness [%] | 99.9 | 92.4 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | Bis-Tris pH 6.5, ammonium sulfate, PEG 3350, VAPOR DIFFUSION, temperature 298K |
