4FGW
Structure of Glycerol-3-Phosphate Dehydrogenase, GPD1, from Sacharomyces Cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 70 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 43 |
| Unit cell lengths | 64.420, 64.420, 198.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.450 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.20750 |
| Structure solution method | Phaser |
| Starting model (for MR) | 1x0v |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.510 | |
| Number of reflections | 29348 | |
| <I/σ(I)> | 14.24 | 3.05 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 12% Polyethylene Glycol 8000, 0.1 M Tris-HCl pH 8.5 and 0.3 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
