4FE9
Crystal Structure of SusF from Bacteroides thetaiotaomicron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2010-12-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97941 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 188.694, 49.148, 83.618 |
Unit cell angles | 90.00, 97.36, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SEMET SUSF |
RMSD bond length | 0.016 |
RMSD bond angle | 1.018 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 46531 | |
<I/σ(I)> | 16.9 | 3.5 |
Completeness [%] | 89.1 | 77.3 |
Redundancy | 2.5 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 292 | 6-12% glycerol, 1.5-2M Na/KPO4, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K |