4FE3
Structure of murine cytosolic 5'-nucleotidase III complexed with uridinine monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-18 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 46.878, 46.878, 287.101 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.740 |
| R-factor | 0.1717 |
| Rwork | 0.169 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bdu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.356 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.080 | 0.555 |
| Number of reflections | 607246 | |
| <I/σ(I)> | 32.17 | 4.8 |
| Completeness [%] | 98.3 | 97.1 |
| Redundancy | 17.9 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 283 | 20% PEG 3350, 100 mM TRIS, 50 mM ammonium acetate , pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
