4FDG
Crystal Structure of an Archaeal MCM Filament
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 169.663, 107.737, 170.205 |
| Unit cell angles | 90.00, 107.89, 90.00 |
Refinement procedure
| Resolution | 47.582 - 4.100 |
| R-factor | 0.3318 |
| Rwork | 0.331 |
| R-free | 0.34130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.768 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_863) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.170 |
| High resolution limit [Å] | 4.100 | 11.090 | 4.100 |
| Rmerge | 0.142 | 0.095 | 0.991 |
| Number of reflections | 45740 | ||
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 99.2 | 93.9 | 99.7 |
| Redundancy | 3.1 | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 277 | pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
