4FCC

Glutamate dehydrogenase from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	105.218, 176.262, 150.250
Unit cell angles	90.00, 89.94, 90.00

Refinement procedure

Resolution	49.653 - 2.000
R-factor	0.1818
Rwork	0.181
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.028
Data reduction software	DENZO
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.653	49.653	2.070
High resolution limit [Å]	1.999	7.740	2.000
Rmerge		0.125	0.590
Total number of observations		23876	107166
Number of reflections	365923
<I/σ(I)>	5.1	4.7	1.2
Completeness [%]	99.2	98.9	92.6
Redundancy	3.7	3.7	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M Bis-Tris/HCl pH 6.5, 0.2 NH4Ac, 19% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K