4FC4
FNT family ion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.300, 101.840, 205.300 |
| Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
| Resolution | 87.750 - 2.400 |
| R-factor | 0.19451 |
| Rwork | 0.192 |
| R-free | 0.23631 |
| Structure solution method | MAD |
| Starting model (for MR) | 3q7k |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.845 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 87.750 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.070 |
| Number of reflections | 135449 |
| <I/σ(I)> | 12 |
| Completeness [%] | 97.4 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 27% PEG 1000, 0.1 M Nacitrate buffer, 0.4 L of 350 mM, C-HEGA-10, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
