4FC2
Crystal structure of mouse poly(ADP-ribose) glycohydrolase (PARG) catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-03-31 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.141, 90.401, 104.696 |
| Unit cell angles | 81.64, 88.41, 89.36 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| R-factor | 0.1759 |
| Rwork | 0.174 |
| R-free | 0.21440 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.123 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 173052 | |
| Completeness [%] | 92.1 | 54.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.2M (NH4)2SO4, 16% PEG3,350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
