4FBC

Structure of mutant RIP from barley seeds in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	133.833, 142.427, 85.236
Unit cell angles	90.00, 127.97, 90.00

Refinement procedure

Resolution	37.733 - 1.700
R-factor	0.1762
Rwork	0.176
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.087
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.053	0.030	0.464
Number of reflections	134051
Completeness [%]	98.0	99.6	96.4
Redundancy	3.8	4.1	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	295	0.1M phosphate-citrate, 0.2M NaCl, 20%(w/v) PEG 8000, pH 4.2, vapor diffusion, hanging drop, temperature 295K