4FAP

ATOMIC STRUCTURES OF THE RAPAMYCIN ANALOGS IN COMPLEX WITH BOTH HUMAN FKBP12 AND FRB DOMAIN OF FRAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1997-02
Detector	SDMS
Spacegroup name	P 21 21 21
Unit cell lengths	45.400, 52.100, 102.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.184
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fap
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	UCSD-system
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Number of reflections	5598
<I/σ(I)>	10.4
Completeness [%]	91.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	8		This particular structure is not described in this paper. *