4FA7
Structure of Recombinant Cytochrome ba3 Oxidase mutant A204F from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.550, 98.210, 94.860 |
Unit cell angles | 90.00, 127.92, 90.00 |
Refinement procedure
Resolution | 74.830 - 2.500 |
R-factor | 0.17174 |
Rwork | 0.169 |
R-free | 0.21969 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s8g |
RMSD bond length | 0.022 |
RMSD bond angle | 2.077 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 74.830 |
High resolution limit [Å] | 2.500 |
Number of reflections | 33684 |
Completeness [%] | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 50mM Sodium Cacodylate, 1.6M NaCl, 40% PEG400, pH 6.5, Lipidic Cubic Phase, temperature 293K |